ABSTRACT
This study aims to develop a UPLC-MS/MS method for simultaneous determination of six pyrrolizidine alkaloids(PAs)--intermedine N-oxide(ImNO), lycopsamine N-oxide(LyNO), seneciphylline(Sp), seneciphylline N-oxide(SpNO), senecionine N-oxide(SnNO), and senkirkine(Sk) in different parts of Emilia sonchifolia. UPLC conditions are as follows: ACQUITY UPLC HSS T3 column(2.1 mm×100 mm, 1.8 μm), mobile phase consisting of 0.05% formic acid and 2.5 mmol·L~(-1) ammonium formate in water(A)-0.05% formic acid and 2.5 mmol·L~(-1) ammonium formate in acetonitrile(B) for gradient elution. MS conditions are as below: electrospray ionization(ESI) in the positive ion mode, multiple reaction monitoring(MRM), and the content of the six PAs was calculated with the external standard method. The results suggested the differences in the six PAs among different parts of E. sonchifolia. Sk was detected in all the four parts, with similar content. SnNO also existed in all the four parts, but the content in roots was significantly higher than that in other parts. Sp and SpNO were found in both roots and flowers, with the content higher in the former than in the later. ImNO and LyNO were only found in leaves, and the content was low. Among the six components detected, ImNO, LyNO, and SpNO were found and determined for the first time, which enriched the toxic components and laid a scientific basis for the quality and safety evaluation of E. sonchifolia.
Subject(s)
Asteraceae , Chromatography, High Pressure Liquid , Chromatography, Liquid , Pyrrolizidine Alkaloids , Tandem Mass SpectrometryABSTRACT
OBJECTIVE: To develop an RP-HPLC method for simultaneous determination of rosmarinic acid, tilianin, luteolin-7-O-β-D-glucuronide, apigenin-7-O-β-D-glucuronide, and diosmetin-7-O-β-D-glucuronide in different parts ofDracocephalum moldevica L. METHODS: The five constituents were measured on a Shim-pack ODS column(4.6 mm×250 mm, 5 μm)with gradient elution of acetonitrile (A)-0.5% formic acid aqeous solution (B) (0-30 min, 17%A;30-60 min, 17%-28%A; 60-70 min, 28%A) at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 330 nm, and the column temperature was maitained at 35℃. RESULTS: The linear ranges of rosmarinic acid, tilianin, luteolin-7-O-β-D-glucuronide, apigenin-7-O-β-D-glucuronide, and diosmetin-7-O-β-D-glucuronide were 4.2-126 μg·mL-1 (r=0.999 2), 7.84-235.2 μg·mL-1 (r=0.999 3), 3.048-91.44 μg·mL-1(r=0.999 4), 1.472-44.16 μg·mL-1(r=0.999 4), and 2.816-84.48 μg·mL-1 (r=0.999 2), respectively. The average recoveries (RSD) of the five compounds were 98.97%(1.03%), 99.90%(0.92%), 99.89% (1.75%), 99.55% (0.98%), and 99.76%(1.19%) (n=6), respectively. CONCLUSION: The developed method is accurate and precise, which can be used for the quality control of different parts of Dracocephalum moldevica L.The RESULTS: of content determination indicate that the five compounds exist in all the parts of Dracocephalum moldevica L., but the mass fractions are obviously different.
ABSTRACT
This study was aimed to establish a method to determine the content of phenolic acids in different parts ofCoptis chinensis, in order to discuss the dynamic change of phenolic acids contents in different parts and growth years ofCoptis chinensis. Contents of total phenolic acid, chlorogenic acid and ferulic acid were determined by ferric chloride-ferricyanatum calcium colorimetric method and HPLC, respectively. The results showed that the content of total phenolic acid inCoptis chinensis was in the range from 98.435 mg·g-1 to 184.456 mg·g-1. The content of chlorogenic acid and ferulic acid was in the range from 0.176 mg·g-1 to 2.227 mg·g-1, and 0.039 mg·g-1 to 0.512 mg·g-1, respectively. It was concluded that the content of phenolic acids in different parts ofCoptis chinensis were significantly different. The phenolic acids contents in different parts of Coptis chinensis reached the highest two years after transplantation, and then it expressed downswing with the increasing of growth period.
ABSTRACT
OBJECTIVE:To analyze the components of the volatile oil in different parts of Lonicera japonica Thunb.from Henan province.METHODS:The volatile oil was extracted from the buds,leaves,stems of L.japonica Thunb.by solid-phase micro-extraction,and the chemical compositions were identified by GC-MS combined with Kvotas retention index.The relative percentage of each constituent was determined by GC area normalization method.RESULTS:Thirty-nine compounds were identified from different parts of L.japonica Thunb.from Henan province,and seven of which were mutual in the buds,leaves,stems of L.japonica Thunb..CONCLUSION:This study serves as a scientific basis for the further development and utilization of L.japonica Thunb..